Western Blot analysis of Rat Brain Membrane showing detection of ~215 kDa Dicer protein using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (12532). Lane 1: MW Ladder. Lane 2: Rat Brain Membrane. Load: 10 µg. Block: 5% milk. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (12532) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~215 kDa.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dicer Monoclonal Antibody, Clone S167-7 (12532). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Dicer Monoclonal Antibody (12532) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Dicer Antibody. (D) Composite.